Basic Investigations
RADIOIMMUNODETECTION OF HUMAN CHORIOCARCINOMA XENOGRAFT IN NUDE MOUSE
Abstract
Objective: To study the effidency of radioimmunodetection in locating the xenograft of human choriocarcinoma in nude mouse.
Methods: Radioimmunodetection was performed using cocktail antibodies of 131Ilabeled mouse anti-human chorioulc gonadotropin monoclonal antibodies to locate the xenograft of human chorlocarcinoma in nude mouse. Radioactivity in different tissues was measured and the tumor/non-tumor ratio was calculated. Normal mouse IgG was used as control IgG.
Results: The accumulation of radioactivity in the xenograft area could be recognized as early as 24 h after the injection of the radiolabelled antibodies. 72--96 h after the injection, the xenograft could be clearly shown. The miniroal shown xenograft was 0.8 cm in diameter. The tumor/non-tumor ratio increased with the time and was obviously higher than that in control group.
Conclusion: Radioimmunodetection can efficiently locate human chorlocarcinoma xenograft in nude mouse.
Methods: Radioimmunodetection was performed using cocktail antibodies of 131Ilabeled mouse anti-human chorioulc gonadotropin monoclonal antibodies to locate the xenograft of human chorlocarcinoma in nude mouse. Radioactivity in different tissues was measured and the tumor/non-tumor ratio was calculated. Normal mouse IgG was used as control IgG.
Results: The accumulation of radioactivity in the xenograft area could be recognized as early as 24 h after the injection of the radiolabelled antibodies. 72--96 h after the injection, the xenograft could be clearly shown. The miniroal shown xenograft was 0.8 cm in diameter. The tumor/non-tumor ratio increased with the time and was obviously higher than that in control group.
Conclusion: Radioimmunodetection can efficiently locate human chorlocarcinoma xenograft in nude mouse.
