Original Article
INHIBITION OF APOPTOSIS BY bcr-abl FUSION GENE IN K562 CELLS
Abstract
Objective: To investigate the effect of bcr-abl fusion gene on CML cell apoptosis.
Methods: Apoptosis of exvivo cultured K562 cells were observed after exposure to synthetic 18 mer antisense oligodeoxynucleotide complementary to the bcr-abl junction (b3a2).
Results: Apoptosis of K562 cells was significantly increased associated with inhibition of bcr-abl expression.
Conclusion: bcr-abl fusion gene formation due to chromosome translocation may be the major mechanism of CML via inhibition of apoptosis.
Methods: Apoptosis of exvivo cultured K562 cells were observed after exposure to synthetic 18 mer antisense oligodeoxynucleotide complementary to the bcr-abl junction (b3a2).
Results: Apoptosis of K562 cells was significantly increased associated with inhibition of bcr-abl expression.
Conclusion: bcr-abl fusion gene formation due to chromosome translocation may be the major mechanism of CML via inhibition of apoptosis.
