TY - JOUR AU - Qin, Sisi AU - Zhang, Baozhen AU - Tian, Wei AU - Gu, Liankun AU - Lu, Zheming AU - Deng, Dajun PY - 2015 TI - Kaiso mainly locates in the nucleus in vivo and binds to methylated, but not hydroxymethylated DNA JF - Chinese Journal of Cancer Research; Vol 27, No 2 (April 23, 2015): Chinese Journal of Cancer Research Y2 - 2015 KW - N2 - Objective: Kaiso is upregulated in many cancers and proposed to bind with both methylated- and unmethylated-DNA in the nucleus as a transcriptional repressor. The objective is to define its subcellular localization in vivo and exact binding DNA sequences in cells. Methods: Compartmentalization of exogenous Kaiso in cells was tracked with enhanced green fluorescence protein (EGFP) tag. The endogenous Kaiso expression in gastric carcinoma tissue was examined with immunohistochemical staining. Kaiso-DNA binding was tested using electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation assay (ChIP). Results: Kaiso mainly localized in the nucleus of cancer and stromal cells in vivo , but remained in the cytoplasm of cultured cells. Most importantly, nuclear Kaiso can bind with the methylated-CGCG-containing sequence in the CDKN2A promoter, but not with the hydroxymethylated-CGCG sequence in HCT116 cells. Conclusions: Kaiso locates mainly in the nucleus in vivo where it binds with the methylated-CGCG sequences, but does not bind with the hydroxymethylated-CGCG sequences. UR - https://cjcr.amegroups.org/article/view/6135