TY - JOUR AU - Liu, Yuan AU - Zeng, Ling-lan AU - Chen, Yan AU - Zhao, Fei AU - Li, Rui AU - Zhang, Chun AU - Wen, Lu PY - 2012 TI - Triptolide Inhibits Cell Growth and Induces G0- G1 Arrest by Regulating P21wap1/cip1 and P27 kip1 in Human Multiple Myeloma RPMI-8226 Cells JF - Chinese Journal of Cancer Research; Vol 22, No 2 (June 29, 2010): Chinese Journal of Cancer Research Y2 - 2012 KW - N2 - Objective: To investigate the effects of triptolide(TPL) on cell growth, cell cycle and the expressions of p21wap1/cip1 and p27kip1. Methods: MTT assay was used to determine the cell viability after triptolide treatment in human multiple myeloma RPMI-8226 cells. The effect on cell cycle distribution was determined by flow cytometry. Semi-quantitative reverse transcription-PCR was used to examine the mRNA expressions of p21wap1/cip1 and p27kip1. The protein expressions of p21 wap1/cip1 and p27kip1 were determined by Western blot. Results: Triptolide of varying concentrations induced cell viability inhibition in dose- and time-related fashion and caused G0- G1 phase arrest of cell cycle progression in RPMI-8226 cells. These effects accompanied with up-modulation of the expressions of p21 wap1/cip1 and p27kip1. Conclusion: These results suggest that triptolide inhibit cell proliferation and cell cycle progression via up-regulating p21wap1/cip1 and p27kip1 and triptolide may exert its anti-cancer activity through this pathway. UR - https://cjcr.amegroups.org/article/view/827