TY - JOUR AU - Li, Jing AU - Wong, Wilfred Hing-Sang AU - Chan, Shing AU - Chim, James Chor-San AU - Cheung, Kenneth Man-Chee AU - Lee, Tsz-Leung AU - Au, Wing-Yan AU - Ha, Shau-Yin AU - Lie, Albert Kwok-Wei AU - Lau, Yu-Lung AU - Liang, Raymond Hin-Suen AU - Chan, Godfrey Chi-Fung PY - 2012 TI - Factors Affecting Mesenchymal Stromal Cells Yield from Bone Marrow Aspiration JF - Chinese Journal of Cancer Research; Vol 23, No 1 (March 30, 2011): Chinese Journal of Cancer Research Y2 - 2012 KW - N2 - Objective: This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells (MSC). Methods: We determined the yield of MSC from bone marrow under different clinical conditions by comparing the MSC colony numbers from: (1) donors of different ages; (2) healthy donors and patients with leukemia; (3) bone marrow aspirated at different time points during marrow harvesting; (4) bone marrow harvested by different needles. Results: During the process of harvesting, the number of MSC significantly decreased with increase number of aspiration, from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated, and 50/ml after 200 ml bone marrow aspirated. The number of MSC retrieved from leukemia patients (99/ml bone marrow) was significantly lower than that of healthy donors (708/ml bone marrow). However, there was no significant difference in growth rate. There was no significant age-related difference of MSC yielded from donors Conclusion: The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting procedure. Bone marrow from donors <55 years was equally good as MSC sources. The autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion. UR - https://cjcr.amegroups.org/article/view/890